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Variations of circulating miRNA in paediatric patients with Heart Failure supported with Ventricular Assist Device: a pilot study

Circulating miRNAs (c-miRNAs) are promising biomarkers for HF diagnosis and prognosis. There are no studies on HF pediatric patients undergoing VAD-implantation. Aims of this study were: to examine the c-miRNAs profile in HF children; to evaluate the effects of VAD on c-miRNAs levels; to in vitro validate putative c-miRNA targets. c-miRNA profile was determined in serum of HF children by NGS before and one month after VAD-implant. The c-miRNA differentially expressed were analyzed by real time-PCR, before and at 4 hrs,1,3,7,14,30 days after VAD-implant. A miRNA mimic transfection study in HepG2 cells was performed to validate putative miRNA targets selected through miRWalk database. Thirteen c-miRNAs were modified at 30 days after VAD-implant compared to pre-VAD at NSG, and, among them, six c-miRNAs were confirmed by Real-TimePCR. Putative targets of the validated c-miRNAs are involved in the hemostatic process. The in vitro study confirmed a down-regulatory effect of hsa-miR-409-3p towards coagulation factor 7 (F7) and F2. Of note, all patients had thrombotic events requiring pump change. In conclusion, in HF children, the level of six c-miRNAs involved in the regulation of hemostatic events changed after 30 days of VAD-treatment. In particular, the lowering of c-miR-409-3p regulating both F7 and F2 could reflect a pro-thrombotic state after VAD-implant.

Scientific Reports, 2020

Autori esterni: Rosetta Ragusa (Institute of Life Sciences, Scuola Superiore Sant’Anna, Pisa, Italy), Arianna Di Molfetta (Department of Cardiothoracic Surgery, Ospedale Pediatrico Bambino Gesù, Rome, Italy), Serena Del Turco (Institute of Clinical Physiology, CNR, Pisa, Italy), Manuela Cabiati (Institute of Clinical Physiology, CNR, Pisa, Italy), Silvia Del Ry (Institute of Clinical Physiology, CNR, Pisa, Italy), Giuseppina Basta (Institute of Clinical Physiology, CNR, Pisa, Italy), Letizia Pitto (Institute of Clinical Physiology, CNR, Pisa, Italy), Antonio Amodeo (Department of Cardiothoracic Surgery, Ospedale Pediatrico Bambino Gesù, Rome, Italy), Maria Giovanna Trivella (Institute of Clinical Physiology, CNR, Pisa, Italy), Milena Rizzo ( Institute of Clinical Physiology, CNR, Pisa, Italy), Chiara Caselli ( Institute of Clinical Physiology, CNR, Pisa, Italy)
Autori IIT:

Tipo: Contributo in rivista ISI
Area di disciplina: Mathematics

File: s41598-020-62757-7.pdf

Attività: Biologia computazionale